N-Cadherin anticorps (CF®405S)
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- Antigène Voir toutes N-Cadherin (CDH2) Anticorps
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
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Reactivité
- Humain, Souris
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp N-Cadherin est conjugé à/à la CF®405S
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Fonction
- Mouse Monoclonal anti-N-Cadherin / Cadherin-2 / CD325 (CDH2/1573), CF405S Conjugate
- Attributs du produit
- This antibody recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. Results aid in the classification of malignant non-carcinomatous neoplasms including mesotheliomas, chordomas, synovial sarcomas, malignant melanomas, epithelioid sarcomas, epithelioid angiosarcomas, clear cell sarcomas as well as serous and endometrioid tumors of the ovary have been demonstrated to be N-cadherin positive, whereas mucinous tumors are negative. Other N-cadherin-positive neoplasms include renal cell carcinomas and some variant breast tumors, including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Recombinant human CDH2 intracellular domain (exact sequence is proprietary)
- Clone
- CDH2-1573
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CDH2 Anticorps primaire
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- Indications d'application
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tis, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
- Autre désignation
- N-Cadherin / Cadherin-2 / CD325 (CDH2 Produits)
- Synonymes
- anticorps CD325, anticorps CDHN, anticorps CDw325, anticorps NCAD, anticorps N-cadherin, anticorps cadherin-2, anticorps cdhn, anticorps ncad, anticorps cdw325, anticorps CDH2, anticorps Cadherin-2, anticorps Ncad, anticorps glo, anticorps lyr, anticorps pac, anticorps wu:fb47h04, anticorps cadherin 2, anticorps cadherin 2 S homeolog, anticorps cadherin 2, type 1, N-cadherin (neuronal), anticorps CDH2, anticorps cdh2.S, anticorps cdh2, anticorps Cdh2
- Sujet
- Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD
- Poids moléculaire
- 130-140 kDa
- ID gène
- 1000, 464829
- UniProt
- P19022
- Pathways
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
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