CD3 epsilon anticorps (AA 23-119) (CF®488A)

N° du produit ABIN6176562

Cet anticorps Souris Monoclonal détecte spécifiquement CD3 epsilon dans FACS, WB, IHC et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD3 epsilon anticorps (AA 23-119) (CF®488A) (ABIN6176562)

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est conjugé à/à la CF®488A

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: C3e-1308

Détail du produit anti-CD3 epsilon anticorps (CF®488A)

Épitope: AA 23-119

Fonction: Mouse Monoclonal anti-CD3e (C3e/1308), CF488A Conjugate

Attributs du produit: This antibody recognizes the ε-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human CD3e fragment (aa 23-119)

Isotype: IgG2b kappa

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Western blotting 1-2 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Jurkat cells. Tonsil and lymph node

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e

Poids moléculaire: 20 kDa

ID gène: 916, 3003

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway