CD3 epsilon anticorps

N° du produit ABIN6176615

Cet anticorps anti-CD3 epsilon Monoclonal Souris (Clone CRIS-7) (ABIN6176615) détecte spécifiquement CD3 epsilon dans FACS, IF, Act et ProA. L’anticorps est réactif avec des échantillons de Humain et Singe.

Aperçu rapide pour CD3 epsilon anticorps (ABIN6176615)

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Activation (Act), Proliferation Assay (ProA)

Clone: CRIS-7

Détail du produit anti-CD3 epsilon anticorps

Fonction: Mouse Monoclonal anti-CD3e (CRIS-7), Purified, BSA-free

Attributs du produit: This antibody recognizes the epsilon-chain of CD3 (Workshop V, Code: CD03.09), which consists of five different polypeptide chains (designated as γ, δ, ε, ζ, and η) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Stimulated human leukocytes

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Induce T cell activation and proliferation
• Optimal dilution for a specific application should be determined by user

Commentaires:

Jurkat cells. Tonsil and lymph node

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e

Poids moléculaire: 20 kDa

ID gène: 916, 3003

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway