KRT17 anticorps

N° du produit ABIN6178097

Détail du produit anti-KRT17 anticorps

Antigène: KRT17 (Keratin 17 (KRT17))

Reactivité: Humain, Rat, Boeuf (Vache), Porc, Chévre

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT17 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Fonction: Mouse Monoclonal anti-Cytokeratin 17 (E3), Purified, BSA-free

Attributs du produit: Cytokeratin 17 (CK17) is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as ?epithelial stem cell? marker because CK17 Ab marks basal cell differentiation. CK17 is expressed in SCLC much higher than in LADC. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: The cytoskeletal fraction of rat colon epithelium

Clone: E3

Isotype: IgG2b kappa

Information d'application

Indications d'application: Immunohistology frozen & formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

T24 cells or Skin. Bladder or Cervix.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur KRT17

Antigène: KRT17 (Keratin 17 (KRT17))

Autre désignation: Cytokeratin 17 (KRT17 Produits)

Synonymes: anticorps K17, anticorps Krt1-17, anticorps PC, anticorps PC2, anticorps PCHC1, anticorps Ka17, anticorps KRT17, anticorps krt16, anticorps si:dkeyp-113d7.7, anticorps KRT14, anticorps keratin 17, anticorps keratin 17 L homeolog, anticorps keratin 17, type I, anticorps keratin, type I cytoskeletal 17, anticorps Krt17, anticorps KRT17, anticorps krt17, anticorps krt17.L, anticorps LOC100737113, anticorps LOC102177275

Sujet: K17, Keratin Type I Cytoskeletal 17, Keratin-17, KRT17, PCHC1

Poids moléculaire: 46 kDa

ID gène: 3872, 2785

UniProt: Q04695