p21 anticorps (CF®647)

N° du produit ABIN6178638

Cet anticorps Souris Monoclonal détecte spécifiquement p21 dans IF, FACS et IHC (f). Il présente une réactivité envers Humain, Souris, Rat, Singe et Chimpanzé.

Aperçu rapide pour p21 anticorps (CF®647) (ABIN6178638)

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivité: Humain, Souris, Rat, Singe, Chimpanzé

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p21 est conjugé à/à la CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HJ21

Détail du produit anti-p21 anticorps (CF®647)

Fonction: Mouse Monoclonal anti-p21 / WAF1 (HJ21), CF647 Conjugate

Attributs du produit: This MAb recognizes a 21 kDa protein, identified as the p21WAF1 tumor suppressor protein. It is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human recombinant p21 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HeLa Cells. Skin, colon, or breast carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur p21

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Autre désignation: p21 / WAF1

Sujet: Cell surface antigen MAX.3, hCD84, Hly9-beta, Leucocyte differentiation antigen CD84, LY9B, Signaling lymphocytic activation molecule 5 (SLAMF5), SLAM family member 5

Poids moléculaire: 21 kDa

ID gène: 1026, 370771

UniProt: P38936

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy