TLR4 anticorps (CF®647)

N° du produit ABIN6181184

Cet anticorps anti-TLR4 est un anticorps Souris Monoclonal détectant TLR4 dans FACS, IF et IHC (f). Adapté pour Humain, Rat, Chien, Porc, Cobaye et Singe.

Aperçu rapide pour TLR4 anticorps (CF®647) (ABIN6181184)

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivité: Humain, Rat, Chien, Porc, Cobaye, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TLR4 est conjugé à/à la CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TLR4-230

Détail du produit anti-TLR4 anticorps (CF®647)

Fonction: Mouse Monoclonal anti-CD284 / TLR4 (TLR4/230), CF647 Conjugate

Attributs du produit: This MAb reacts with human Toll-like receptor 2 (TLR4). It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human TLR4 protein

Isotype: IgG2a kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HeLa, PC12, human peripheral blood cells. Dendritic cells, macrophage and monocytes in tonsil, placenta and adrenal.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur TLR4

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Autre désignation: CD284 / TLR4

Sujet: ARMD10, CD284, hToll, TLR4, TOLL, Toll Endotoxin Hyporesponsiveness, Toll-like receptor 4

Poids moléculaire: 95-120 kDa

ID gène: 7099, 174312

UniProt: O00206

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins