FBXO5 anticorps (AA 1-250)

N° du produit ABIN6186001

Détail du produit anti-FBXO5 anticorps

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Épitope: AA 1-250

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FBXO5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Fonction: Mouse Monoclonal anti-EMI1 (EMI1/1176), Purified, BSA-free

Attributs du produit: This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Clone: EMI1-1176

Isotype: IgG2a kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Western blotting 1-2 μg/mL
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HeLa, 293 or HepG2 cells. Ovarian carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur FBXO5

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Autre désignation: EMI1 (FBXO5 Produits)

Synonymes: anticorps EMI1, anticorps FBX5, anticorps Fbxo31, anticorps 2510044I10Rik, anticorps C85305, anticorps Emi1, anticorps emi1, anticorps fc65h02, anticorps wu:fc65h02, anticorps wu:fe06e07, anticorps wu:fz79f03, anticorps zgc:136397, anticorps zgc:158541, anticorps fbx5, anticorps Fbxo5, anticorps ACYPI007854, anticorps fbxo5, anticorps F-box protein 5, anticorps F-box only protein 5, anticorps F-box protein 5 L homeolog, anticorps F-box protein 5 S homeolog, anticorps FBXO5, anticorps Fbxo5, anticorps fbxo5, anticorps fbxo5.L, anticorps fbxo5.S

Sujet: Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31

Poids moléculaire: 56 kDa

ID gène: 26271, 520506

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases