ZAP70 anticorps (AA 1-254) (CF®568)

N° du produit ABIN6189284

Cet anticorps anti-ZAP70 est un anticorps Souris Monoclonal détectant ZAP70 dans FACS, IF et IHC (f). Adapté pour Humain.

Aperçu rapide pour ZAP70 anticorps (AA 1-254) (CF®568) (ABIN6189284)

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZAP70 est conjugé à/à la CF®568

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 2F3-2

Détail du produit anti-ZAP70 anticorps (CF®568)

Épitope: AA 1-254

Fonction: Mouse Monoclonal anti-ZAP70 (2F3.2), CF568 Conjugate

Attributs du produit: ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70

Isotype: IgG2a kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Jurkat cells. Tonsil or lymph node.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur ZAP70

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP70

Sujet: Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70  kDa

Poids moléculaire: 70 kDa

ID gène: 7535, 234569

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway