CD3 epsilon anticorps (Activation Site) (FITC)

N° du produit ABIN6240811

Cet anticorps anti-CD3 epsilon est un anticorps Souris Monoclonal détectant CD3 epsilon dans ICFC. Adapté pour Humain et Souris.

Aperçu rapide pour CD3 epsilon anticorps (Activation Site) (FITC) (ABIN6240811)

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est conjugé à/à la FITC

Application: Intracellular Flow Cytometry (ICFC)

Clone: APA1-1

Détail du produit anti-CD3 epsilon anticorps (FITC)

Épitope: Activation Site

Fonction: Anti-CD3 epsilon (activation epitope) FITC

Specificité: The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Réactivité croisée (Details): Human, Mouse

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Purified human CD3 proteins isolated from thymus

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3 epsilon

Sujet: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 epsilon chain, T3E, IMD18

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway