CD43 anticorps
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- Antigène Voir toutes CD43 (SPN) Anticorps
- CD43 (SPN) (Sialophorin (SPN))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD43 est non-conjugé
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purification
- Protein G affinity chromatography
- Immunogène
- Myeloblastic KG1 cells were used as the immunogen.
- Clone
- DF-T1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product SPN Anticorps primaire
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- Indications d'application
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Western Blot: 0.5-1 μg/mL
Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Antigène
- CD43 (SPN) (Sialophorin (SPN))
- Autre désignation
- CD43 (SPN Produits)
- Synonymes
- anticorps CD43, anticorps GALGP, anticorps GPL115, anticorps LSN, anticorps A630014B01Rik, anticorps Cd43, anticorps Galgp, anticorps Ly-48, anticorps Ly48, anticorps Lsn, anticorps Lsn1, anticorps gpL115, anticorps sialophorin, anticorps SPN, anticorps Spn
- Sujet
- This antibody recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. 70-90 % of T-cell lymphomas and 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with CD20 antibody, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with CD20 and CD42 antibody argues against a reactive process and favors a diagnosis of lymphoma.
- ID gène
- 6693
- UniProt
- P16150
- Pathways
- Regulation of Leukocyte Mediated Immunity
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