G-CSF anticorps

N° du produit ABIN6251958

Détail du produit anti-G-CSF anticorps

Antigène: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp G-CSF est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogène: Human recombinant full-length protein was used as the immunogen for the G-CSF antibody.

Clone: PRPN2-1

Isotype: IgG1

Information d'application

Indications d'application: Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the G-CSF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur G-CSF

Antigène: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Autre désignation: G-CSF (CSF3 Produits)

Synonymes: anticorps CSF3, anticorps G-CSF, anticorps gcsf, anticorps Csfg, anticorps MGI-IG, anticorps C17orf33, anticorps CSF3OS, anticorps GCSF, anticorps Gcsf, anticorps colony stimulating factor 3, anticorps colony stimulating factor 3 (granulocyte) a, anticorps colony stimulating factor 3 (granulocyte), anticorps CSF3, anticorps csf3a, anticorps Csf3

Sujet: This mAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF's, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF's share 73 % sequence identity at the amino acid level.

ID gène: 1440

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization