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MYH11 anticorps

MYH11 Reactivité: Humain, Rat, Cat, Poulet, Boeuf (Vache), Chien, Cobaye, Porc, Lapin IF, FACS, IHC (p) Hôte: Souris Monoclonal SPM201 unconjugated
N° du produit ABIN6251989
  • Antigène Voir toutes MYH11 Anticorps
    MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))
    Reactivité
    • 37
    • 20
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    Humain, Rat, Cat, Poulet, Boeuf (Vache), Chien, Cobaye, Porc, Lapin
    Hôte
    • 32
    • 8
    Souris
    Clonalité
    • 20
    • 20
    Monoclonal
    Conjugué
    • 23
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Cet anticorp MYH11 est non-conjugé
    Application
    • 13
    • 12
    • 10
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G affinity chromatography
    Immunogène
    Human uterus extract was used as the immunogen for the SM-MHC antibody.
    Clone
    SPM201
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product MYH11 Anticorps primaire
  • Indications d'application
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    The stated application concentrations are suggested starting amounts. Optimal dilution of the SM-MHC antibody should be determined by the researcher.

    1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Antigène
    MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))
    Autre désignation
    SM-MHC (MYH11 Produits)
    Synonymes
    anticorps AV071570, anticorps SM1, anticorps SM2, anticorps smMHC, anticorps AAT4, anticorps FAA4, anticorps SMHC, anticorps SMMHC, anticorps myosin, anticorps myh11, anticorps myosin, heavy polypeptide 11, smooth muscle, anticorps myosin heavy chain 11, anticorps myosin, heavy chain 11, smooth muscle, anticorps myosin, heavy chain 11a, smooth muscle, anticorps Myh11, anticorps MYH11, anticorps myh11a
    Sujet
    Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.
    ID gène
    4629
    UniProt
    P35749
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