alpha Fetoprotein anticorps

N° du produit ABIN6252177

Détail du produit anti-alpha Fetoprotein anticorps

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivité: Humain, Porc, Chien, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Fetoprotein est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Specificité: Does not react with cow, cat, mouse, rat.

Purification: Protein G affinity chromatography

Immunogène: Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.

Clone: C3

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the AFP antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Aliquot and Store at 2-8C. Avoid freez-thaw cycles.

Détails sur alpha Fetoprotein

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Autre désignation: Alpha Fetoprotein (AFP Produits)

Synonymes: anticorps FETA, anticorps HPAFP, anticorps alpha fetoprotein, anticorps alpha-fetoprotein, anticorps AFP, anticorps Afp

Sujet: This antibody recognizes an oncofetal glycoprotein with a single chain of 70  kDa, which is identified as Alpha fetoprotein (AFP). The AFP antibody is highly specific and shows no cross-reaction with other oncofetal antigens or serum albumin. Alpha fetoprotein is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. AFP antibody has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

ID gène: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process