Myogenin anticorps
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- Antigène Voir toutes Myogenin (MYOG) Anticorps
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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Reactivité
- Humain, Souris, Rat, Porc, Cat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Myogenin est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purification
- Protein G affinity chromatography
- Immunogène
- Human myogenin recombinant protein was used as the immunogen for this antibody.
- Clone
- MGN185
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MYOG Anticorps primaire
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- Indications d'application
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Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Myogenin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Antigène
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
- Autre désignation
- Myogenin (MYOG Produits)
- Synonymes
- anticorps cb553, anticorps zgc:100763, anticorps LOC446037, anticorps myf4, anticorps Xmyog, anticorps myogenin, anticorps LOC100136471, anticorps LOC100224062, anticorps LOC100303673, anticorps MYF4, anticorps bHLHc3, anticorps myo, anticorps myf-4, anticorps myogenin, anticorps myogenin (myogenic factor 4), anticorps MYOG, anticorps myog, anticorps LOC100303673, anticorps Myog
- Sujet
- Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Myogenin antibody labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.
- ID gène
- 4656
- UniProt
- P15173
- Pathways
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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