CD3 epsilon anticorps

N° du produit ABIN6252412

L’anticorps anti-CD3 epsilon Polyclonal Lapin est utilisé pour la détection de CD3 epsilon dans des échantillons de Humain, Souris, Rat, Porc, Boeuf (Vache), Cheval, Lapin, Cobaye, Poulet, Chien, Mouton, Hamster, Cat, Cryptomys amatus (Mole rat), Elephant, Grenouille, Opossum et Primate. Il a été validé pour FACS, WB et IP.

Aperçu rapide pour CD3 epsilon anticorps (ABIN6252412)

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Cheval, Lapin, Cobaye, Poulet, Chien, Mouton, Hamster, Cat, Cryptomys amatus (Mole rat), Elephant, Grenouille, Opossum, Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-CD3 epsilon anticorps

Purification: Protein A purified CD3e antibody

Immunogène: Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry: use the CD3e antibody at 0.5-1 μg/million cells

WB: 0.5-1.0 μg/mL

IP: 1-2 μg/500 μg protein lysate



The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e

Sujet: This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway