CPS1 anticorps

N° du produit ABIN6252613

Détail du produit anti-CPS1 anticorps

Antigène: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Reactivité: Humain, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CPS1 est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the CPS1 antibody.

Clone: CPS1-1022

Isotype: IgG1

Information d'application

Indications d'application: Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the CPS1 antibody should be determined by the researcher.

1.Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CPS1

Antigène: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Autre désignation: CPS1 (CPS1 Produits)

Synonymes: anticorps CPSASE1, anticorps cps, anticorps 4732433M03Rik, anticorps CPS, anticorps D1Ucla3, anticorps Cps1, anticorps carbamoyl-phosphate synthase 1, anticorps carbamoyl-phosphate synthase 1 L homeolog, anticorps carbamoyl-phosphate synthetase 1, anticorps peptidase M20 domain containing 1, anticorps CPS1, anticorps Cps1, anticorps cps1.L, anticorps cps1, anticorps PM20D1

Sujet: This mAb recognizes a protein of 165  kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

ID gène: 1373

UniProt: P31327

Pathways: Response to Growth Hormone Stimulus, Cellular Glucan Metabolic Process