TYR anticorps

N° du produit ABIN6252820

Détail du produit anti-TYR anticorps

Antigène: TYR (Tyrosinase (TYR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TYR est non-conjugé

Application: Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the Tyrosinase antibody.

Clone: OCA1-812

Isotype: IgG2a kappa

Information d'application

Indications d'application: ELISA (order Ab without BSA)

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the Tyrosinase antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, or 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur TYR

Antigène: TYR (Tyrosinase (TYR))

Autre désignation: Tyrosinase (TYR Produits)

Synonymes: anticorps sandy, anticorps zgc:109705, anticorps TYR, anticorps TYRO, anticorps tyr, anticorps LOC100136546, anticorps CMM8, anticorps OCA1A, anticorps OCAIA, anticorps SHEP3, anticorps albino, anticorps c, anticorps skc35, anticorps C, anticorps tyrosinase, anticorps TYRosinase, anticorps tyrosinase S homeolog, anticorps tyr, anticorps tyr-5, anticorps tyr.S, anticorps TYR, anticorps Celal_3121, anticorps Halhy_4121, anticorps Mesop_2736, anticorps LOC100136546, anticorps Tyr

Sujet: Recognizes a cluster of proteins between 70-80  kDa, identified as tyrosinase. Occasionally a minor band at 55  kDa is also detected. This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.

ID gène: 7299

UniProt: P14679