PRKG1 anticorps (C-Term)
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- Antigène Voir toutes PRKG1 Anticorps
- PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))
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Épitope
- C-Term
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Reactivité
- Humain, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PRKG1 est non-conjugé
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Application
- Western Blotting (WB), ELISA
- Specificité
- PRKG1 Antibody detects endogenous levels of total PRKG1.
- Homologie
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogène
- A synthesized peptide derived from human PRKG1, corresponding to a region within C-terminal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product PRKG1 Anticorps primaire
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- Indications d'application
- WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Date de péremption
- 12 months
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- Antigène
- PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))
- Autre désignation
- PRKG1 (PRKG1 Produits)
- Synonymes
- anticorps prkg1, anticorps zgc:63955, anticorps Pkgi, anticorps cGk1, anticorps 1, anticorps PKG, anticorps PRKG1B, anticorps PRKGR1B, anticorps cGK, anticorps cGK 1, anticorps cGK1, anticorps cGKI, anticorps cGKI-BETA, anticorps cGKI-alpha, anticorps CGKI, anticorps cGKI-beta, anticorps AW125416, anticorps Gm19690, anticorps Prkg1b, anticorps Prkgr1b, anticorps Prkg1beta, anticorps protein kinase, cGMP-dependent, type Ia, anticorps protein kinase, cGMP-dependent, type I, anticorps protein kinase, cGMP-dependent, type 1, anticorps prkg1a, anticorps PRKG1, anticorps prkg1, anticorps Prkg1
- Sujet
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Description: Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca2+ release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca2+ levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation, decreasing contraction, controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.
Gene: PRKG1
- Poids moléculaire
- 76 kDa
- ID gène
- 5592
- UniProt
- Q13976
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