PPP1R12A anticorps (pThr853)

N° du produit ABIN6288239

Détail du produit anti-PPP1R12A anticorps

Antigène: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Épitope: AA 590-670, pThr853

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PPP1R12A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: MYPT1 is a protein encoded by the PPP1R12A gene which is approximately 115,2 kDa. MYPT1 is localised to the cytoplasm. It is involved in the activation of cAMP-dependent PKA, beta-adrenergic signalling, vascular smooth muscle contraction and actin nucleation by ARP-WASP complex. It regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho and is also a key regulator of protein phosphatase 1C. MYPT1 is expressed in striated muscles, specifically in type 2a fibres. Mutations in the PPP1R12A gene may result in infratentorial cancer and medullomyoblastoma. STJ90822 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous MYPT1 protein which only binds about T853 when T853 is phosphorylated.

Specificité: Phospho-MYPT1 (T853) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.

Attributs du produit: Rabbit polyclonal to Phospho-MYPT1 (T853).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human MYPT1 around the phosphorylation site of T853.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000

Commentaires:

Expressed in striated muscles, specifically in type 2a fibers (at protein level).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C, and avoid repeat freeze-thaw cycles.

Détails sur PPP1R12A

Antigène: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Autre désignation: MYPT1 (PPP1R12A Produits)

Synonymes: anticorps 1200015F06Rik, anticorps 5730577I22Rik, anticorps AA792106, anticorps AV099298, anticorps D10Ertd625e, anticorps Mypt1, anticorps M130, anticorps MBS, anticorps MYPT1, anticorps M110, anticorps MBSP, anticorps protein phosphatase 1, regulatory (inhibitor) subunit 12A, anticorps protein phosphatase 1 regulatory subunit 12A, anticorps protein phosphatase 1, regulatory subunit 12A, anticorps Ppp1r12a, anticorps PPP1R12A

Poids moléculaire: 130 kDa

ID gène: 4659

UniProt: O14974

Pathways: M Phase