TLR4 anticorps (Internal Region)

N° du produit ABIN6655892

N° du produit (Fournisseur): 600-401-g52

Cet anticorps anti-TLR4 est un anticorps Lapin Polyclonal détectant TLR4 dans WB, FACS, IHC, IF et FM. Adapté pour Humain.

Aperçu rapide pour TLR4 anticorps (Internal Region) (ABIN6655892)

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TLR4 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-TLR4 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-g52

Fournisseur: Rockland

Fonction: TLR4 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with TRL 4 from Human and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR4 Antibody was purified by affinity chromatography.

Stérilité: Sterile filtered

Immunogène: TRL4 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against synthetic peptide corresponding to an internal region of human TLR4.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 1:50

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1-3 μg/mL

Commentaires:

Anti-TLR4 Antibody has been tested in WB, IHC, IF microscopy and Flow Cytometry. Expect a band approximately ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag). Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.5 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur TLR4

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Autre désignation: TLR4

Sujet:

Synonyms: ARMD10, CD284, TLR 4, TOLL, Toll like receptor 4

Background: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediatiors of LPS signaling. The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90 kDa.

Gene Name: TRL4

ID gène: 7099

NCBI Accession: NP_612564

UniProt: O00206

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins