TLR4 anticorps

N° du produit ABIN6656855

N° du produit (Fournisseur): 200-301-i25

Cet anticorps anti-TLR4 est un anticorps Souris Monoclonal détectant TLR4 dans WB, FACS, IHC, ELISA, IF, FM, ChIP et DB. Adapté pour Humain.

Aperçu rapide pour TLR4 anticorps (ABIN6656855)

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TLR4 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Clone: 76B357-1

Détail du produit anti-TLR4 anticorps

N° du produit (Fournisseur): 200-301-i25

Fournisseur: Rockland

Fonction: TLR4 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR4 from human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR4 Antibody was purified by Protein G chromatography.

Immunogène: TLR4 Antibody was produced in mice prepared by repeated immunizations with amino acids corresponding to an internal sequence in human TLR4.

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 5 μg/mL

Flow_Cytometry_Dilution: 0.5-2.0 μg/10^6 cells

IF_Microscopy_Dilution: 1:10-1:2000

Western_Blot_Dilution: 1-3 μg/mL

Commentaires:

Anti-TLR4 Antibody has been tested in WB, ChIP, DB, ELISA, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Dual ISH-IHC, ELISA(Capture), and Knockdown/Knockout. Expect a band approximately 95kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur TLR4

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Autre désignation: TLR4

Sujet:

Synonyms: hToll, Toll-like receptor 4, CD284

Background: Anti-TLR4 CD284 antibody detects human TLR4 CD284. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa. Anti-TLR4 CD284 antibody is ideal for investigators involved in cytokines and growth factor research.

Gene Name: TLR4

ID gène: 7099

NCBI Accession: NP_003257

UniProt: O00206

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins