MEK1 anticorps (pThr386)

N° du produit ABIN6657389

N° du produit (Fournisseur): 600-401-d73

Cet anticorps anti-MEK1 Polyclonal Lapin (ABIN6657389) détecte spécifiquement MEK1 dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour MEK1 anticorps (pThr386) (ABIN6657389)

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MEK1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-MEK1 anticorps

Épitope: pThr386

N° du produit (Fournisseur): 600-401-d73

Fournisseur: Rockland

Fonction: MEK1 phospho T386 Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Purification: Anti-MEK1 pT386 antibodies are prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogène: Anti-MEK1 pT386 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr 386 conjugated to KLH.

Isotype: IgG

Information d'application

Indications d'application: Optional[Neutralization_Dilution]: 1:1000

Commentaires:

Anti-MEK1 pT386 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to the MEK1 protein phosphorylated at Thr386 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur MEK1

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation: MEK1

Sujet:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1

Background: MEK1 pT386 antibody is directed against human MEK 1 phosphorylated against Thr386. MEK also known as MAP Kinase Kinase and MKK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386. MEK1 pT386 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Gene Name: MAP2K1

ID gène: 5604

UniProt: Q02750

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling