c-MYC anticorps (C-Term)

N° du produit ABIN6657589

N° du produit (Fournisseur): 100-401-224

L’anticorps Lapin Polyclonal anti-c-MYC a été validé pour WB et ELISA. Il convient pour détecter c-MYC dans des échantillons de Souris.

Aperçu rapide pour c-MYC anticorps (C-Term) (ABIN6657589)

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp c-MYC est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-c-MYC anticorps

Épitope: C-Term

N° du produit (Fournisseur): 100-401-224

Fournisseur: Rockland

Fonction: c-Myc Antibody

Réactivité croisée (Details): Anti-c-myc is highly specific for c-myc and shows no cross-reaction with N-myc or L-myc proteins.

Purification: This antiserum is directed against human c-myc and is useful in determining its presence in various assays.

Stérilité: Sterile filtered

Immunogène: This whole rabbit serum was prepared by repeated immunizations with c-myc peptide corresponding to amino acids KHKLEQLRNSGA which map to the last 12 amino acids of the mouse

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 1:500- 1:2,000

Other: User Optimized

Commentaires:

Anti-c-myc has been tested by western blot. This antibody reacts with human and mouse c-myc protein, which is a nuclear protein with a molecular weight of approximately 66 kDa.  This antiserum detects c-myc by immunoblotting assays in both overexpressed and endogenous systems.   Reactivity in other immunoassays has not yet been fully determined although perinuclear and cytoplasmic staining has been reported.  The recommended positive controls are SK-BR-3 cells (adenocarcinoma, breast, malignant pleural effusion, human).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur c-MYC

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Autre désignation: c-Myc

Sujet:

Synonyms: rabbit anti-c-Myc Antibody, Myc proto-oncogene protein antibody, Proto-oncogene c-Myc antibody, Transcription factor p64 antibody, Class E basic helix-loop-helix protein 39 antibody

Background: c-myc is involved in the control of cell proliferation and differentiation at the transition from S --> G₁ phase for a normal cell.  Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain), 2 (k light chain), or 22 (l light chain) is described in Burkitt's lymphoma and other B-cell lympho-proliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.  Abnormal expression can take many forms including transduction, insertional activation, translocation, and amplification.

Gene Name: Myc, c-myc

ID gène: 17869

NCBI Accession: NP_001170823

UniProt: P01108

Pathways: Signalisation p53, Cycle Cellulaire, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, L'effet Warburg