TLR4 anticorps

N° du produit ABIN6657874

N° du produit (Fournisseur): 200-501-i26

L’anticorps Rat Monoclonal anti-TLR4 a été validé pour WB, FACS, IP et IF. Il convient pour détecter TLR4 dans des échantillons de Souris.

Aperçu rapide pour TLR4 anticorps (ABIN6657874)

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TLR4 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: MTS510

Détail du produit anti-TLR4 anticorps

N° du produit (Fournisseur): 200-501-i26

Fournisseur: Rockland

Fonction: TLR4 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR4 from mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR4 Antibody was purified by Protein G chromatography.

Immunogène: TLR4 Antibody was produced in rats prepared by repeated immunizations with the Ba/F3 cell line expressing mouse protein TLR4.

Isotype: IgG2a

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 2-5 μg/10^6 cells

Western_Blot_Dilution: 1:500-1:1000

Commentaires:

Anti-TLR4 Antibody has been tested in ICC/IF, Flow (CS), and IP. Expect a band approximately 95kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur TLR4

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Autre désignation: TLR4

Sujet:

Synonyms: hToll, Toll-like receptor 4, CD284

Background: Anti-TLR4 CD284 Antibody detects human TLR4. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa. Anti-TLR4 CD284 Antibody is ideal for invedtigators invovled in cytokines and growth factor research.

Gene Name: TLR4

ID gène: 21898

NCBI Accession: NP_067272

UniProt: Q9QUK6

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins