AKT2 anticorps (C-Term)

N° du produit ABIN6719267

L’anticorps anti-AKT2 Monoclonal Souris est utilisé pour la détection de AKT2 dans des échantillons de Humain. Il a été validé pour WB, IF, FACS et ICC.

Aperçu rapide pour AKT2 anticorps (C-Term) (ABIN6719267)

Antigène: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AKT2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 10C6

Détail du produit anti-AKT2 anticorps

Épitope: C-Term

Fonction: Anti-AKT2 Antibody (monoclonal, 10C6)

Séquence: DRYDSLGLLE LDQRTHFPQF SYSASIRE

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: different from the related mouse sequence by two amino acids,and from the related rat sequence by one amino acid.

Attributs du produit: Anti-AKT2 Antibody.(monoclonal, 10C6)-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human AKT2, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

Isotype: IgG1

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Commentaires:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur AKT2

Antigène: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Autre désignation: AKT2

Sujet:

Background: AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. This gene is mapped to 19q13.2. AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.

Gene Full Name: AKT serine/threonine kinase 2

Poids moléculaire: 56 kDa

ID gène: 208

UniProt: P31751

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Signalisation TLR, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling