PDK4 anticorps (N-Term)

N° du produit ABIN6736421

Cet anticorps Lapin Polyclonal détecte spécifiquement PDK4 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Cheval, Porc, Lapin, Roussette (Chauve-souris) et Singe.

Aperçu rapide pour PDK4 anticorps (N-Term) (ABIN6736421)

Antigène: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reactivité: Humain, Cheval, Porc, Lapin, Roussette (Chauve-souris), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDK4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PDK4 anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Human PDK4

Homologie: Percent identity by BLAST analysis: Human, Rabbit, Horse, Pig (100%) Mouse, Rat, Bovine, Guinea pig (92%) Dog (91%) Chicken (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from N-Terminus of human PDK4 (Q16654, NP_002603). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Bat, Rabbit, Horse, Pig, Platypus (100%), Mouse, Rat, Bovine, Guinea pig (92%), Dog (91%), Lizard (90%), Turkey, Chicken (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur PDK4

Antigène: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Autre désignation: PDK4

Sujet: Name/Gene ID: PDK4
Subfamily: PDK/BCKDK
Family: Protein Kinase

Synonyms: PDK4, PDHK4

ID gène: 5166

NCBI Accession: NP_002603

UniProt: Q16654

Pathways: Signalisation PI3K-Akt, Signalisation Hedgehog, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg