ATP6V0A2 anticorps (AA 72-121)

N° du produit ABIN6741595

Détail du produit anti-ATP6V0A2 anticorps

Antigène: ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))

Épitope: AA 72-121

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATP6V0A2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human ATP6V0A2

Homologie: Percent identity by BLAST analysis: Human, Rat (100%) Dog, Horse (92%) Pig (91%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa72-121 of human ATP6V0A2 (Q9Y487, NP_036595). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat (100%), Monkey, Galago, Elephant, Dog, Bat, Horse (92%), Pig (91%), Bovine, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur ATP6V0A2

Antigène: ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))

Autre désignation: ATP6V0A2 (ATP6V0A2 Produits)

Synonymes: anticorps A2, anticorps ARCL, anticorps ARCL2A, anticorps ATP6A2, anticorps ATP6N1D, anticorps J6B7, anticorps RTF, anticorps STV1, anticorps TJ6, anticorps TJ6M, anticorps TJ6S, anticorps VPH1, anticorps WSS, anticorps 8430408C20Rik, anticorps AI385560, anticorps ATP6a2, anticorps AW489264, anticorps Atp6n1d, anticorps Atp6n2, anticorps C76904, anticorps ISF, anticorps SHIF, anticorps Stv1, anticorps TJ6s, anticorps Tj6, anticorps V-ATPase 116 kDa, anticorps V-ATPase a2, anticorps Cc1-3, anticorps J6b7, anticorps atp6v0a2, anticorps si:ch211-199i18.4, anticorps si:ch211-106a19.2, anticorps ATPase H+ transporting V0 subunit a2, anticorps ATPase, H+ transporting, lysosomal V0 subunit A2, anticorps ATPase, H+ transporting, lysosomal V0 subunit a2a, anticorps ATPase, H+ transporting, lysosomal V0 subunit a2b, anticorps ATP6V0A2, anticorps Atp6v0a2, anticorps atp6v0a2a, anticorps atp6v0a2b

Sujet: Name/Gene ID: ATP6V0A2
Subfamily: ATPase - V type
Family: Transporter

Synonyms: ATP6V0A2, A2V-ATPase, ARCL, ARCL2A, ATP6A2, ATP6N1D, RTF, STV1, V-ATPase 116 kDa, TJ6S, WSS, J6B7, TJ6, TJ6M, V-ATPase 116 kDa isoform a2, VPH1

ID gène: 23545

NCBI Accession: NP_036595

UniProt: Q9Y487

Pathways: Transition Metal Ion Homeostasis, Proton Transport