GAPDH anticorps (AA 1-335)

N° du produit ABIN678458

Détail du produit anti-GAPDH anticorps

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Épitope: AA 1-335

Reactivité: Humain, Souris, Rat, Lapin, Porc, Poulet, Boeuf (Vache), Différentes espèces

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Poulet, Boeuf (Vache), Humain, Souris, Porc, Lapin, Rat, Différentes espèces

Purification: Purified by Protein A.

Immunogène: GAPDH protein of rabbit

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-GAPDH (ABIN678458)

Zhang, Wang, Xie, Chen, Sinkemani, Yu, Wang, Mao, Wu: "Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence." dans: Oncotarget, Vol. 9, Issue 2, pp. 2175-2192, (2018) (PubMed).

Sun, Zhang, Tang, Yang, Chu, Zhao: "SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer." dans: International journal of nanomedicine, Vol. 12, pp. 2657-2672, (2017) (PubMed).

Tan, Shi, Ba, Lin, Tang, Zeng, Zhang: "miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy." dans: International journal of molecular medicine, Vol. 37, Issue 4, pp. 1030-8, (2016) (PubMed).

Jia, Song, Yang, Ma, Li, Lu, Cao, Zhang, Zhu, Wang, Leng, Cao, Du, Xu: "Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats." dans: Molecular medicine reports, Vol. 13, Issue 6, pp. 4627-35, (2016) (PubMed).

Wang, Jia, Chen, Wang, Li: "Osthole shows the potential to overcome P-glycoprotein‑mediated multidrug resistance in human myelogenous leukemia K562/ADM cells by inhibiting the PI3K/Akt signaling pathway." dans: Oncology reports, Vol. 35, Issue 6, pp. 3659-68, (2016) (PubMed).

Xiong, Luo, He, Xi, Lu, Li, Liu, Guo: "Up-regulation of miR-138 inhibits hypoxia-induced cardiomyocyte apoptosis via down-regulating lipocalin-2 expression." dans: Experimental biology and medicine (Maywood, N.J.), Vol. 241, Issue 1, pp. 25-30, (2016) (PubMed).

Yang, Yao, Qian, Wei, Wu, Wang: "Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium." dans: Cellular and molecular life sciences : CMLS, Vol. 72, Issue 24, pp. 4849-66, (2016) (PubMed).

Hou, Liu, Chai, Yu, Duan, Hu, Yin, Wang, Zhuang, Fan, Chu, Ma: "Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein." dans: Molecular medicine reports, Vol. 12, Issue 2, pp. 2521-8, (2015) (PubMed).

Zhang, Shi, Lin, Ba, Cui: "MicroRNA-216a enhances the radiosensitivity of pancreatic cancer cells by inhibiting beclin-1-mediated autophagy." dans: Oncology reports, Vol. 34, Issue 3, pp. 1557-64, (2015) (PubMed).

Shao, Qian, Wang, Yan, Hu, Wang, Wang: "Construction of the plasmid coding for the expression of the EGFP-M-IL-2((88)Arg, (125)Ala) fusion protein and the anti-tumor effects exerted by the fusion protein in HeLa-60 cells." dans: Oncology letters, Vol. 9, Issue 6, pp. 2729-2735, (2015) (PubMed).

Zhang, Feng, Tao, DE, Chang, Hu: "Increased stathmin expression strengthens fear conditioning in epileptic rats." dans: Biomedical reports, Vol. 3, Issue 1, pp. 28-32, (2015) (PubMed).

Song, Xiao, Fu, Huang, Wang, Zhang, Yang: "Increased expression of the pluripotency markers sex-determining region Y-box 2 and Nanog homeobox in ovarian endometriosis." dans: Reproductive biology and endocrinology : RB&E, Vol. 12, pp. 42, (2014) (PubMed).

Yan, Yang, Li, Duan, Wu, Qian, Li, Wang: "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." dans: Journal of cardiovascular pharmacology, Vol. 64, Issue 4, pp. 310-7, (2014) (PubMed).

Steenblock, Heckel, Czupalla, Espírito Santo, Niehage, Sztacho, Hoflack: "The Cdc42 guanine nucleotide exchange factor FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts." dans: The Journal of biological chemistry, Vol. 289, Issue 26, pp. 18347-59, (2014) (PubMed).

Li, McFadden, Morgan, Cui, Hu, Wan, Zeng: "All-trans retinoic acid regulates the expression of the extracellular matrix protein fibulin-1 in the guinea pig sclera and human scleral fibroblasts." dans: Molecular vision, Vol. 16, pp. 689-97, (2010) (PubMed).

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: Gapdh (GAPDH Produits)

Synonymes: anticorps G3PD, anticorps GAPD, anticorps Gapd, anticorps BEST:GH12586, anticorps CG12055, anticorps Dmel\\CG12055, anticorps GA3PDH, anticorps GADPH, anticorps GAP, anticorps GAPDH, anticorps GAPDH I, anticorps GAPDH-1, anticorps GAPDH1, anticorps GAPDHI, anticorps Gapdh, anticorps Gapdh-1, anticorps Gapdh43E, anticorps gadph, anticorps gapdh, anticorps gapdh-1, anticorps gh12586, anticorps cb609, anticorps gapd, anticorps mg:bb02e05, anticorps wu:fb33a10, anticorps wu:ft80f05, anticorps KNC-NDS6, anticorps g3pd, anticorps G3PDH, anticorps glyceraldehyde-3-phosphate dehydrogenase, anticorps Glyceraldehyde 3 phosphate dehydrogenase 1, anticorps glyceraldehyde-3-phosphate dehydrogenase S homeolog, anticorps glyceraldehyde-3-phosphate dehydrogenase, type I, anticorps olfactory receptor 8K3, anticorps GAPDH, anticorps Gapdh, anticorps Gapdh1, anticorps gapdh, anticorps gapdh.S, anticorps gapDH, anticorps LOC100404960, anticorps LOC614985

Sujet:

Synonyms: G3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, CDABP47, OK/SW-cl.12

Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

ID gène: 2597

UniProt: P04406