EGFR anticorps (Extracellular Domain) (CF®488A)

N° du produit ABIN6934272

L’anticorps anti-EGFR Monoclonal Souris est utilisé pour la détection de EGFR dans des échantillons de Humain. Il a été validé pour IF et FACS.

Aperçu rapide pour EGFR anticorps (Extracellular Domain) (CF®488A) (ABIN6934272)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est conjugé à/à la CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: GFR450

Détail du produit anti-EGFR anticorps (CF®488A)

Épitope: Extracellular Domain

Specificité: This MAb recognizes a protein of 170 kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Aucune reactivité croisée: Souris

Purification: Purified by Protein A/G

Immunogène: Recombinant extracellular domain of human EGFR protein

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: A431 cells, Breast or Bladder cancer.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR

Poids moléculaire: ~170kDa (wild type) and ~145kDa (vIII variant)

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins