p21 anticorps

N° du produit ABIN6936905

Cet anticorps Souris Monoclonal détecte spécifiquement p21 dans WB, IF et ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour p21 anticorps (ABIN6936905)

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p21 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Clone: AC8

Détail du produit anti-p21 anticorps

Fonction: p21WAF1 (Tumor Suppressor Protein) Antibody

Specificité: This MAb recognizes a 21 kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

Aucune reactivité croisée: Souris, Rat (Rattus)

Réactivité croisée (Details): Human. Does not react with Mouse or Rat.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human CDKN1A protein

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA), Western Blot (1-2 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein), Immunofluorescence (1-2 μg/mL) Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF7 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur p21

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Autre désignation: CDKN1A

Sujet:

Activating Fragment 1, CAP20, CDK-interacting protein 1, CDKI, CDKN1, CDKN1A, CIP1, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA6, Melanoma Differentiation Associated Protein 6, p21Cip1/Waf1, PIC1, SDI1, SLC12A9, Wild type p53 activated fragment 1 (WAF1)

HepG-2, HeLa or MCF7 cells.

Poids moléculaire: 21kDa

ID gène: 1026, 370771

UniProt: P38936

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy