COL7A1 anticorps

N° du produit ABIN6937011

Détail du produit anti-COL7A1 anticorps

Antigène: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp COL7A1 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Réactivité croisée (Details): Human. Sheep. Goat. Horse. Guinea Pig. Cow.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human Collagen VII protein

Clone: LH7-2

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur COL7A1

Antigène: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Autre désignation: COL7A1 (COL7A1 Produits)

Synonymes: anticorps COL7A1, anticorps EBD1, anticorps EBDCT, anticorps EBR1, anticorps AW209154, anticorps collagen type VII alpha 1 chain, anticorps collagen, type VII, alpha 1, anticorps COL7A1, anticorps col7a1, anticorps Col7a1

Sujet: Alpha 1 type VII collagen, Collagen 7, Collagen alpha 1(VII) chain, Collagen type VII alpha 1, Collagen VII alpha 1 polypeptide Col7a1, EBD1, EBDCT, EBR1, Long-chain (LC) collagen,Collagen VII
Cellular localisation: Secreted. Extracellular matrix. Basement Membrane.

Poids moléculaire: 290kDa

ID gène: 1294, 476218

UniProt: Q02388