COL7A1 anticorps

N° du produit ABIN6939168

Détail du produit anti-COL7A1 anticorps

Antigène: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Reactivité: Humain, Boeuf (Vache), Chévre, Cobaye, Cheval, Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp COL7A1 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Staining Methods (StM)

Specificité: Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human Collagen VII protein

Clone: LH7-2

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (0.5-1.0 μg/mL)Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur COL7A1

Antigène: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Autre désignation: COL7A1 (COL7A1 Produits)

Synonymes: anticorps COL7A1, anticorps EBD1, anticorps EBDCT, anticorps EBR1, anticorps AW209154, anticorps collagen type VII alpha 1 chain, anticorps collagen, type VII, alpha 1, anticorps COL7A1, anticorps col7a1, anticorps Col7a1

Poids moléculaire: 290kDa

ID gène: 1294

UniProt: Q02388