EGFR anticorps (AA 344-492)

N° du produit ABIN6937129

Cet anticorps anti-EGFR Monoclonal Souris (Clone SPM341) (ABIN6937129) détecte spécifiquement EGFR dans WB, IHC, IF et FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour EGFR anticorps (AA 344-492) (ABIN6937129)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: SPM341

Détail du produit anti-EGFR anticorps

Épitope: AA 344-492

Fonction: EGFR (Epidermal Growth Factor Receptor) Antibody

Specificité: This MAb recognizes a protein of 170 kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment of human EGFR protein (around aa 344-492) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A431 cells. Placenta, Breast, Colon or Bladder cancer.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR

Sujet:

Erbb1, ERBB1, Errp, HER1, mENA, PIG61, Proto-oncogene c-ErbB-1, Receptor Tyrosine Protein Kinase, ErbB1, Urogastrone, wa2, Wa5

A431 cells. Placenta, Breast, Colon or Bladder cancer.

Poids moléculaire: ~170kDa (wild type) and ~145kDa (vIII variant)

ID gène: 1956, 488293

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins