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Myc Tag anticorps (AA 408-439)

Reactivité: Humain IF, FACS, IHC (f) Hôte: Souris Monoclonal 9E10-3 unconjugated

N° du produit ABIN6937982

Fiche de données

Antigène Tous les produits Myc Tag

Myc Tag
Épitope
20

18

12

8

6

3

2

1
AA 408-439
Reactivité
22

5

1
Humain
Hôte
77

43

6

5

5

3

1

1
Souris
Anticorps secondaires appropriés
Clonalité
79

50

10
Monoclonal
Conjugué
63

13

10

7

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp Myc Tag est non-conjugé
Application
108

51

36

35

25

23

23

17

15

11

11

6

6

4

4

4

4

3

2

2

1

1

1

1

1

1
Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité

It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

Aucune reactivité croisée

Poulet, Souris, Rat (Rattus)

Réactivité croisée (Details)

Human. Does not react with Mouse, Rat or Chicken.

Purification

1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène

A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.

Clone

9E10-3

Isotype

IgG1 kappa
anti-Myc Tag antibody
WB, IP, ELISA, IF Hôte: Souris Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
Reactivité: Humain WB, IP, IHC (p), FACS Hôte: Souris Monoclonal 9E10 unconjugated

anti-Myc Tag antibody
WB, IP, ELISA Hôte: Lapin Polyclonal unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
Reactivité: Humain FACS Hôte: Souris Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
Reactivité: Humain WB, IP, IHC (p), FACS Hôte: Souris Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA, IF Hôte: Souris Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA Hôte: Souris Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF Hôte: Souris Monoclonal 3B8 unconjugated

anti-Myc Tag antibody (Biotin)
WB, IP, ELISA, IHC Hôte: Lapin Polyclonal Biotin

anti-Myc Tag (AA 410-419) antibody
WB Hôte: Chèvre Polyclonal unconjugated

Indications d'application

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: HL-60 cells. Cervical Carcinoma.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur

Azide free

Stock

-20 °C,-80 °C

Stockage commentaire

Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption

24 months
Antigène

Myc Tag

Abstract

Myc Tag Produits

Classe de substances

Tag

Sujet

Class E basic helix-loop-helix protein 39 (bHLHe39), MRTL, Myc2, Niard, Nird, Proto-oncogene c-Myc, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog,c-Myc Oncoprotein
Cellular localisation: Nuclear

Poids moléculaire

62-64kDa

ID gène

4609, 202453

UniProt

P01106