TIGIT anticorps (AA 22-141)

N° du produit ABIN6938572

Détail du produit anti-TIGIT anticorps

Antigène: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Épitope: AA 22-141

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TIGIT est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation). It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa 22-141) of human TIGIT protein (exact sequence is proprietary)

Clone: TIGIT-3017

Isotype: IgG2c kappa

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4, Prostate or Colon Carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur TIGIT

Antigène: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Autre désignation: TIGIT (TIGIT Produits)

Synonymes: anticorps VSIG9, anticorps VSTM3, anticorps WUCAM, anticorps ENSMUSG00000071552, anticorps Vstm3, anticorps RGD1563191, anticorps T-cell immunoreceptor with Ig and ITIM domains, anticorps T cell immunoreceptor with Ig and ITIM domains, anticorps TIGIT, anticorps Tigit

Sujet: T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain containing 9 (VSIG9), V-set and transmembrane domain containing 3 (VSTM3), Washington University cell adhesion molecule (WUCAM),TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer)
Cellular localisation: Cell Surface

Poids moléculaire: 26kDa

ID gène: 201633, 421750

Pathways: Cancer Immune Checkpoints