ZAP70 anticorps (AA 247-382)

N° du produit ABIN6938798

Détail du produit anti-ZAP70 anticorps

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Épitope: AA 247-382

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZAP70 est non-conjugé

Application: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Specificité: ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells.Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa 247-382) of human ZAP70 protein (exact sequence is proprietary)

Clone: ZAP70-2046

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells. Tonsil or lymph node.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur ZAP70

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP70 (ZAP70 Produits)

Synonymes: anticorps SRK, anticorps STCD, anticorps STD, anticorps TZK, anticorps ZAP-70, anticorps 70kDa, anticorps AI327364, anticorps Srk, anticorps mrtle, anticorps mur, anticorps zap70, anticorps MGC80670, anticorps MGC88962, anticorps ZAP70, anticorps zgc:110383, anticorps zeta chain of T-cell receptor associated protein kinase 70, anticorps putative tyrosine-protein kinase zap-70, anticorps zeta-chain (TCR) associated protein kinase, anticorps zeta chain of T cell receptor associated protein kinase 70, anticorps zeta-chain (TCR) associated protein kinase 70kDa, anticorps zeta chain of T cell receptor associated protein kinase 70kDa L homeolog, anticorps zeta chain of T cell receptor associated protein kinase 70kDa, anticorps tyrosine-protein kinase ZAP-70, anticorps ZAP70, anticorps Smp_149470, anticorps Zap70, anticorps zap70.L, anticorps zap70, anticorps LOC707555, anticorps LOC100342021, anticorps LOC100390922, anticorps LOC100542877

Sujet: Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70 kDa,ZAP70 (Chronic Lymphocytic Leukemia Marker)
Cellular localisation: Cell Surface and Cytoplasmic

Poids moléculaire: 70kDa

ID gène: 7535, 234569

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway