EGFR anticorps

N° du produit ABIN6939289

Cet anticorps anti-EGFR Monoclonal Souris (Clone B1D8) (ABIN6939289) détecte spécifiquement EGFR dans FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour EGFR anticorps (ABIN6939289)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Flow Cytometry (FACS)

Clone: B1D8

Détail du produit anti-EGFR anticorps

Fonction: EGFR (Epidermal Growth Factor Receptor) Antibody

Specificité: This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGF'induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Microsomes from A431 cells.

Isotype: IgG2a, kappa

Information d'application

Indications d'application:

Positive Control: A431 cells, Breast or Bladder cancer.

Known Application: Affinity Purification (For coupling, order Ab without BSA), Blocks Ligand-induced Activation of EGFR, Inhibits Proliferation of A431 Cells (Order Ab without sodium azide), Arrests Tumor Growth in vivo (Order Ab without BSA &Azide), Immunoprecipitation (1-2 μg/500 μg protein lysate), Flow Cytometry (0.5-1 μg/million cells), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR

Sujet:

Erbb1, ERBB1, Errp, HER1, mENA, PIG61, Proto-oncogene c-ErbB-1, Receptor Tyrosine Protein Kinase, ErbB1, Urogastrone, wa2, Wa5

A431 cells, Breast or Bladder cancer.

Poids moléculaire: ~170kDa (wild type) and ~145kDa (vIII variant)

ID gène: 1956, 488293

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins