HLA-DRA anticorps

N° du produit ABIN6939635

Cet anticorps Souris Monoclonal détecte spécifiquement HLA-DRA dans FACS et IF. Il présente une réactivité avec des échantillons de Humain et Singe.

Aperçu rapide pour HLA-DRA anticorps (ABIN6939635)

Antigène: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HLA-DRA est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: IPO-10

Détail du produit anti-HLA-DRA anticorps

Fonction: HLA-DRA (MHC II) Antibody

Specificité: MAb IPO-10 defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells). The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected. '

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Spleen cells of a patient with hairy cell leukemia (Daudi cells)

Isotype: IgG3, kappa

Information d'application

Indications d'application:

Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur HLA-DRA

Antigène: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Autre désignation: HLA-DRA

Sujet:

DR alpha chain, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DR alpha chain, HLA DRA, HLA DRA1, HLA-DRA, HLADRA1, Major histocompatibility complex class II DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA

Daudi or HuT78 cells. Tonsil or lymph node., Ramos

Poids moléculaire: 36kDa

ID gène: 3122, 520048

UniProt: P01903

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response