MCM6 anticorps (AA 228-368)

N° du produit ABIN6940016

L’anticorps anti-MCM6 Monoclonal Souris est utilisé pour la détection de MCM6 dans des échantillons de Humain, Rat, Cobaye et Hamster. Il a été validé pour IHC et WB.

Aperçu rapide pour MCM6 anticorps (AA 228-368) (ABIN6940016)

Antigène: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Reactivité: Humain, Rat, Cobaye, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCM6 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: MCM6-2999

Détail du produit anti-MCM6 anticorps

Épitope: AA 228-368

Fonction: MCM6 (Proliferation Marker) Antibody

Specificité: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa 228-368) of human MCM6 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Positive Control: Human skin or squamous cell carcinoma (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MCM6

Antigène: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Autre désignation: MCM6

Sujet:

DNA replication licensing factor MCM6, MCM6 minichromosome maintenance deficient 6, MIS5 homolog, p105MCM

A-431, Human skin or squamous cell carcinoma (IHC). Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney or Guinea pig Kidney

Poids moléculaire: 105kDa

ID gène: 4175, 444118

UniProt: Q14566

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA