TRAF1 anticorps (AA 73-219)

N° du produit ABIN6940805

Cet anticorps Souris Monoclonal détecte spécifiquement TRAF1 dans IHC et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour TRAF1 anticorps (AA 73-219) (ABIN6940805)

Antigène: TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TRAF1 est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: TRAF1-2770

Détail du produit anti-TRAF1 anticorps

Épitope: AA 73-219

Specificité: This MAb recognizes a protein of 52 kDa, which is identified as TNFR1 (TNFR-associated factor 1). CD30-positive lymphoproliferations of the skin comprise 30 % of all primary cutaneous T-cell lymphomas (CTCLs). Besides borderline cases this group includes lymphomatoid papulosis (LyP) and primary cutaneous anaplastic large T-cell lymphoma (cALCL). Although the two entities overlap clinically, histopathologically, immunopathologically and genetically, they differ considerably in their prognosis. In particular, common feature of both cases is histologically the presence of atypical lymphoid CD30-positive T blasts and genetically a clonal T-cell-receptor rearrangement. However, both cases differ considerably in their clinical course: Lesions of LyP regress spontaneously, whereas those of cALCL persist and may progress and spread. Moreover, LyP patients do not benefit from an aggressive radio- and/or chemotherapeutic approach, in contrast to patients with cALCL. Besides, LyP and cALCL differ strongly in the expression of TRAF1 (tumor necrosis factor receptor (TNFR)-associated factor 1), a component of TNFR signaling: Whereas tumor cells of most LyP cases (ca. 84 %) show a strong TRAF1 expression, tumor cells of cALCL reveal TRAF1 expression in only a few cases (ca. 7 %). Antibody to TRAF1 is highly useful for the differentiation of LyP and cALCL in patients with cutaneous CD30-positive lymphoproliferations.

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment of human TRAF1 protein (around aa 73-219) (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HeLa or 293T cells. Hodgkin s Lymphoma (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur TRAF1

Antigène: TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))

Autre désignation: TRAF1

Poids moléculaire: 52kDa

ID gène: 7185

UniProt: Q13077

Pathways: Signalisation NF-kappaB, Apoptose, Cell-Cell Junction Organization, Asymmetric Protein Localization