CD3 epsilon anticorps (Cytoplasmic Domain)

N° du produit ABIN6941021

Cet anticorps anti-CD3 epsilon est un anticorps Souris Monoclonal détectant CD3 epsilon dans FACS, WB et IF. Adapté pour Humain, Rat, Porc, Boeuf (Vache), Cheval, Lapin, Cobaye et Poulet.

Aperçu rapide pour CD3 epsilon anticorps (Cytoplasmic Domain) (ABIN6941021)

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain, Rat, Porc, Boeuf (Vache), Cheval, Lapin, Cobaye, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: PC3-188A

Détail du produit anti-CD3 epsilon anticorps

Épitope: AA 156-168, Cytoplasmic Domain

Specificité: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Purification: Purified by Protein A/G

Immunogène: A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3-ε chain

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Jurkat cells. Tonsil and lymph node.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL for 60 minutes at RT), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3E

Poids moléculaire: 20kDa

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway