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Blood Group Lewis Y anticorps

Reactivité: Humain FACS, IF, IHC, StM Hôte: Souris Monoclonal A70-A-A9 unconjugated
N° du produit ABIN6933805
  • Antigène
    Blood Group Lewis Y
    Reactivité
    Humain
    Hôte
    • 11
    Souris
    Clonalité
    • 11
    Monoclonal
    Conjugué
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Inconjugué
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
    Specificité
    This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
    Purification
    Purified by Protein A/G
    Immunogène
    Live Ls174T cells (human colon carcinoma cell line)
    Clone
    A70-A-A9
    Isotype
    IgG1 kappa
  • Indications d'application

    Positive Control: Lung, Colon, Bladder or Hepatocellular Carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    Blood Group Lewis Y
    Autre désignation
    Lewis Y (Tumor Marker)
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