Human Papilloma Virus 16 E1, E4 (HPV-16 E1, E4) (AA 36-41) anticorps

N° du produit ABIN6941450

Détail du produit

Antigène: Human Papilloma Virus 16 E1, E4 (HPV-16 E1, E4)

Épitope: AA 36-41

Reactivité: Human Papillomavirus 16 (HPV-16)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Recombinant human HPV16 E1/E4 fragment (aa 36-41).

Clone: HPV16 E1-E4

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HPV-16 infected cells. Cervical tissue.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: Human Papilloma Virus 16 E1, E4 (HPV-16 E1, E4)

Autre désignation: HPV16 E1/E4 (Human Papilloma Virus 16)

Classe de substances: Viral Protein

Sujet: The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a 'high-risk' sexually-transmitted HPV that can lead to cervical, anal, vulvar, head, neck, and penile cancer. The HPV16E1/E4 protein is expressed abundantly in cells supporting viral DNA amplification, but is lost during malignant progression. HPV16E1/E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. HPV16E1/E4 also interacts with cyclin A and Cdk2 during the G2 phase of the cell cycle, and this association may increase the efficiency with which HPV16E1/E4 is able to prevent mitotic entry. HPV16E1/E4 also associates with keratin intermediate filaments and causes the network to collapse.

Poids moléculaire: 16kDa