TNF alpha anticorps (PromoFluor-488 Premium)

N° du produit ABIN6963901

Cet anticorps anti-TNF alpha Monoclonal Souris conjugué à PromoFluor-488 Premium (Clone MT15B15) (ABIN6963901) détecte spécifiquement TNF alpha dans FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour TNF alpha anticorps (PromoFluor-488 Premium) (ABIN6963901)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est conjugé à/à la PromoFluor-488 Premium

Application: Flow Cytometry (FACS)

Clone: MT15B15

Détail du produit anti-TNF alpha anticorps (PromoFluor-488 Premium)

Specificité: Native and recombinant TNF-α

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogène: Recombinant human TNF-alpha

Isotype: IgG1

Information d'application

Indications d'application: Detection of human TNF-α producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of TNF-α by flow cytometry in viable human PBMC. Cells were stimulated for 3 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were stained with an anti-CD3-PE antibody, fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with antibody MT15B15-PF488P (right panel). Matched isotype control antibody (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Date de péremption: 18 months

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNFA

ID gène: 7124

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome