PLEKHM1 anticorps (N-Term)

N° du produit ABIN6991224

Détail du produit anti-PLEKHM1 anticorps

Antigène: PLEKHM1 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 1 (PLEKHM1))

Épitope: AA 50-100, N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PLEKHM1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: PLEKHM1 Antibody is affinity chromatography purified via peptide column.

Immunogène: PLEKHM1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human PLEKHM1. The immunogen is located within amino acids 50 - 100 of PLEKHM1.

Isotype: IgG

Information d'application

Indications d'application: PLEKHM1 antibody can be used for detection of PLEKHM1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PLEKHM1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: PLEKHM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur PLEKHM1

Antigène: PLEKHM1 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 1 (PLEKHM1))

Autre désignation: PLEKHM1 (PLEKHM1 Produits)

Synonymes: anticorps AP162, anticorps B2, anticorps OPTB6, anticorps BC038943, anticorps D330036J23Rik, anticorps pleckstrin homology and RUN domain containing M1, anticorps pleckstrin homology domain containing, family M (with RUN domain) member 1, anticorps PLEKHM1, anticorps Plekhm1

Sujet: PLEKHM1 Antibody: PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, a group of proteins containing a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain. It was identified through segregation analysis as a cause of osteopetrosis in humans. PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7. Monocytes from a patient homozygous for a mutated form of PLEKHM1differentiated into osteoclasts normally, but failed to form ruffled borders and showed little evidence of bone resorbtion when cultured on dentine discs. Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.

ID gène: 9842

UniProt: Q9Y4G2