TNF alpha anticorps (AA 77-197)

N° du produit ABIN7426703

Cet anticorps anti-TNF alpha Monoclonal Souris (Clone 1-1#) (ABIN7426703) détecte spécifiquement TNF alpha dans WB, IHC, ICC et IP. L’anticorps est réactif avec des échantillons de Macaque rhésus.

Aperçu rapide pour TNF alpha anticorps (AA 77-197) (ABIN7426703)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Macaque rhésus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 1-1#

Détail du produit anti-TNF alpha anticorps

Épitope: AA 77-197

Fonction: Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

Specificité: The antibody is a mouse monoclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.

Réactivité croisée: Humain

Purification: Protein A + Protein G affinity chromatography

Immunogène: Recombinant Tumor Necrosis Factor Alpha (TNFa) corresdonding to Val77~Gly197 Plus RGLSAGERVIDSALRSICPTISTLPSSGQV with N-terminal His Tag

Isotype: IgG2a kappa

Information d'application

Indications d'application: Western blotting: 0.5-2 μg/mL,
Immunohistochemistry: 5-20 μg/mL,
Immunocytochemistry: 5-20 μg/mL,
Optimal working dilutions must be determined by end user.

Commentaires:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Date de péremption: 24 months

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNFa

Sujet: DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2

UniProt: P48094

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome