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BRAF anticorps (AA 457-717)

Cet anticorps anti-BRAF est un anticorps Souris Monoclonal détectant BRAF dans WB, IHC, ICC et IP. Adapté pour Humain.
N° du produit ABIN7427177

Aperçu rapide pour BRAF anticorps (AA 457-717) (ABIN7427177)

Antigène

Voir toutes BRAF Anticorps
BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivité

  • 204
  • 73
  • 53
  • 6
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Humain

Hôte

  • 185
  • 20
  • 2
Souris

Clonalité

  • 161
  • 46
Monoclonal

Conjugué

  • 120
  • 15
  • 11
  • 10
  • 8
  • 8
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp BRAF est non-conjugé

Application

  • 115
  • 110
  • 83
  • 31
  • 23
  • 19
  • 13
  • 13
  • 11
  • 10
  • 8
  • 7
  • 3
  • 3
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone

C5
  • Épitope

    • 17
    • 12
    • 11
    • 10
    • 9
    • 8
    • 8
    • 8
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 457-717

    Fonction

    Monoclonal Antibody to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF)

    Specificité

    The antibody is a mouse monoclonal antibody raised against BRAF. It has been selected for its ability to recognize BRAF in immunohistochemical staining and western blotting.

    Purification

    Protein A + Protein G affinity chromatography

    Immunogène

    Recombinant B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) corresdonding to Ile457~Leu717 with N-terminal His Tag

    Isotype

    IgG1 kappa
  • Indications d'application

    Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.47 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    24 months
  • Antigène

    BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

    Autre désignation

    BRAF

    Sujet

    B-raf 1, BRAF1, RAFB1, V-Raf Murine Sarcoma Viral Oncogene Homolog B1

    UniProt

    P15056

    Pathways

    Signalisation MAPK, Signalisation RTK, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
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