TNF alpha anticorps (AA 79-234)
Aperçu rapide pour TNF alpha anticorps (AA 79-234) (ABIN7430006)
Antigène
Voir toutes TNF alpha AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 79-234
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Fonction
- Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
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Specificité
- The antibody is a rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogène
- Recombinant Tumor Necrosis Factor Alpha (TNFa) corresdonding to Arg79~Leu234 with N-terminal His Tag
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Isotype
- IgG
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Indications d'application
- Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.
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Commentaires
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Date de péremption
- 24 months
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Autre désignation
- TNFa
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Sujet
- DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
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UniProt
- P13296
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Pathways
- Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
Antigène
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