RPA3 anticorps

N° du produit ABIN7118404

Détail du produit anti-RPA3 anticorps

Antigène: RPA3 (Replication Protein A3, 14kDa (RPA3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPA3 est non-conjugé

Application: ELISA

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: replication protein A3, 14kDa

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Date de péremption: 12 months

Détails sur RPA3

Antigène: RPA3 (Replication Protein A3, 14kDa (RPA3))

Autre désignation: RPA3 (RPA3 Produits)

Synonymes: anticorps REPA3, anticorps 14kDa, anticorps C330026P08Rik, anticorps im:6908405, anticorps zgc:56310, anticorps zgc:86697, anticorps rpa3, anticorps MGC82610, anticorps RPA3, anticorps repa3, anticorps replication protein A3, anticorps replication protein A3 S homeolog, anticorps replication protein A3 L homeolog, anticorps RPA3, anticorps Rpa3, anticorps rpa3, anticorps rpa3.S, anticorps rpa3.L

Sujet: Synonyms:REPA3, RPA14 Background:As part of the heterotrimeric replication protein A complex(RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage(PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response(PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair(PubMed:7697716). Plays also a role in base excision repair(BER), probably through interaction with UNG(PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA(PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C(RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange(PubMed:19996105).

ID gène: 6119

UniProt: P35244

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA