TNF alpha anticorps (AA 1-233)

N° du produit ABIN7120393

Cet anticorps Souris Monoclonal détecte spécifiquement TNF alpha dans WB, ELISA et IHC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour TNF alpha anticorps (AA 1-233) (ABIN7120393)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 2A9

Détail du produit anti-TNF alpha anticorps

Épitope: AA 1-233

Fonction: TNF alpha antibody

Purification: Protein A+G purification

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène:

Immunogen sequence: 1-233aa

Immunogen: tumor necrosis factor(TNF superfamily, member 2)

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1:500-1:5000, IHC: 1:200-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF alpha

Sujet:

Synonyms: Tumor necrosis factor|Cachectin|TNF-alpha|Tumor necrosis factor ligand superfamily member 2 (TNF-a)|Tumor necrosis factor, membrane form Alternative names: N-terminal fragment (NTF)|Intracellular domain 1 (ICD1)|Intracellular domain 2 (ICD2)|C-domain 1|C-domain 2|Tumor necrosis factor, soluble form|TNF|TNFA|TNFSF2

Background: TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor(TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme(TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Poids moléculaire: 22 kDa

ID gène: 7124

UniProt: P01375

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome